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Bacteria Classification By Gram Staining
Part 1 of Essay
THE AMERICAN UNIVERSITY IN CAIRO
BIOLOGY DEPARTMENT
SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1
Objective:
To test a sample of laboratory prepared bacteria and categorise it
according to Christian’s gram positive and gram negative classes and also by
viewing it under a high powered microscope and oil immersions; cl ....
Part 2 of Essay .... b. Iodine Solution c. 95% Ethyl Alcohol d.
Safranin e. Distilled Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil
Procedure:
A. Preparation :
1. Bacteria is cultivated on agar jelly in an incubator at 25°C for 24 hours. 2.
Obtain a microscope slide and with a toothpick, smear a thin coat of the
bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet
and leave standing for 20 seconds 4. Wash off the stain with distilled water;
drain and blot off the excess with bibulous pa ....
Word count: 751 | Approximate pages: 3
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